血管内皮生长因子165(酵母来源)(VEGF165)(Human)

产品货号：	{[ object.datas[0].sub_number ]}
旧货号：	{[ object.datas[0].number_old ]}
产品规格：
价格：	￥{[ object.datas[0].price\|number:0 ]} 询价
数量：	－＋

产品描述 :

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells,which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels (collateral circulation) to bypass blocked vessels.Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (a.a.) in length.VEGF production can be induced in cells that are not receiving enough oxygen.VEGF165 appears to be the most abundant and potent isoform,followed by VEGF121 and VEGF189. Recombinant human VEGF165 contains 166 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 88 % a.a. with corresponding regions of mouse and rat, 96 % with porcine, 95 % with canine, and 93 % with feline, equine andbovine VEGF, respectively.

表达系统 :
Yeast

溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS,pH 7.4,with 0.02% Tween-20.

存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

分子量:

Theoretically as a disulfide-linked homodimeric protein, the product consists of two 166 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least three

bands with molecular weights ranging from 20-31 kDa in SDS-PAGE under reducing conditions.

内毒素:
Less than 1 EU/μg of rHuVEGF₁₆₅ ,Yeast-derived as determined by LAL method.

产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:
The ED₅₀ determined by a cell proliferation assay usinghuman umbilical vein endothelial cells (HUVEC) is between 1.0-8.0 ng/ml.

产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列：

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

产品纯度:
> 97% by SDS-PAGE and HPLC analyses.